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. 2013 Jun 20;8(6):e63461. doi: 10.1371/journal.pone.0063461

Figure 2. Similar A3A editing of 5MeC and C in vitro.

Figure 2

A) The CER target sequence is nested between two PCR primer targets (black arrows). Every 5MeC site has matched non-methylated equivalent to allow comparisons. B) 3DPCR recovery of A3A edited CER DNA. A3A-myc-His was purified from HEK293T cells as described [18]. The asterisks denote the PCR products cloned and sequenced. C) A selection of deaminated CER sequences with the number of mutant sequences shown to the right. D) Site specific cytidine and 5-methylcytidine deamination frequencies as a function of A3A concentration.