Figure 2. Effects of TKIs on phosphorylated KIT with variant KIT mutations.

(a) COS-1 cells transfected with KIT single mutants were starved and treated with indicated doses of IM, SU, and nilotinib for 30 minutes. The total expression and degree of phosphorylation of KIT were determined by western blot analysis. (b) Inhibitory ratios at Css (IR_Css) of IM, SU, and nilotinib, as the red arrow pointed, on KIT single mutations were determined by quantification of phosphorylated KIT/total KIT and estimated from the western blot in (a). The data are expressed as the mean ± SE of three independent experiments.