Figure 5. Effects and antitumor activities of TKI on GIST48 cells.

(a) GIST48 cells were starved and treated with indicated doses of TKIs for 30 minutes respectively. The total expression and degree of phosphorylation of KIT were determined by western blot analysis. (b) Inhibitory ratios at Css (IR_Css) of TKIs, as the red arrow pointed, on mutated KIT of GIST48 were determined by quantification of phosphorylated KIT/total KIT and estimated from the western blot in (a). (c) Antitumor activities of TKIs against GIST48 cells were preformed with TKIs as indicated doses for 3 days respectively. The cell viabilities were determined by comparing each data to untreated control. (d) Survival ratios at Css of TKIs against GIST48 were estimated from (c). The data are expressed as the mean ± SE of three independent experiments.