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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Acquir Immune Defic Syndr. 2013 Aug 1;63(4):428–437. doi: 10.1097/QAI.0b013e31828ac85f

TABLE 1.

Measurement of Host Response mRNA Expression Levels in HiLNs by Real-time RT-PCR

UI (n=6) SIV (n=10) AIDS (n=6) Fold-change MW P-value
Median Range Median Range Median Range SIV/UI AIDS/UI SIV/UI AIDS/UI
Chemokines: Increased
CXCL9a 2.4b 1.0 – 10.6 25.4b 5.0 – 44.8 28.4b 5.0 – 44.8 10.6 11.8 0.003 0.008
CXCL10 1.0 0.9 – 2.7 5.4 2.1 – 13.8 5.4 2.1 – 7.2 5.4 5.4 0.003 0.008
CXCL11 1.4 0.9 – 4.9 9.0 3.5 – 52.3 8.2 5.6 – 19.2 6.4 5.9 0.002 0.005
CCL3 1.8 1.0 – 2.9 7.7 3.1 – 19.0 9.5 7.1 – 14.7 4.3 5.3 0.001 0.005
CCL4 1.8 0.8 – 3.1 3.3 1.9 – 7.7 4.0 2.2 – 7.7 1.8 2.2 nsc ns
CCL5 1.3 0.5 – 2.0 3.5 0.4 – 6.3 3.8 0.4 – 6.3 2.7 2.9 0.034 ns
CCL18 1.5 0.8 – 4.9 5.1 1.2 – 40.1 9.9 1.7 – 40.1 3.4 6.6 0.033 0.015
CCL23 1.2 0.6 – 1.7 2.5 1.0 – 12.2 2.7 1.2 – 5.4 2.1 2.2 ns ns
Chemokines: Decreased
CCL17 1.3 1.0 – 1.8 0.3 0.02 – 0.9 0.3 0.02 – 0.8 0.2 0.2 0.001 0.005
CCL21 0.7 0.6 – 1.0 0.1 0.0 – 0.5 0.1 0.0 – 0.3 0.1 0.1 0.001 0.005
CCL25 1.1 0.6 - 1.6 0.4 0.01 - 1.2 0.4 0.04 - 1.2 0.4 0.4 0.02 0.045
CXCL12 1.2 0.5 - 1.9 0.6 0.3 - 1.3 0.5 0.3 - 1.3 0.5 0.4 0.02 0.045
CCL20 1.0 0.4 - 5.9 0.5 0.1 - 2.0 0.4 0.1 - 0.8 0.5 0.4 ns 0.045
Chemokine Receptors: Increasedd
CCR5 1.6 0.2 - 2.5 3.3 0.3 - 8.7 5.3 0.3 - 8.7 2.1 3.3 ns ns
CXCR3 2.0 1.0 - 2.8 4.1 0.1 - 7.1 4.1 0.1 - 5.7 2.0 2.0 0.04 ns
Chemokine Receptors: Decreasedd
CCR4 1.3 0.6 - 2.6 0.4 0.3 - 1.6 0.4 0.3 - 0.7 0.3 0.3 0.011 0.008
CCR6 0.9 0.3 - 1.1 0.3 0.1 - 0.5 0.2 0.1 - 0.3 0.3 0.2 0.005 0.01
CCR7 1.2 1.0 - 2.6 0.5 0.03 - 2.5 0.5 0.03 - 1.1 0.4 0.4 0.011 0.008
CCR8 0.6 0.03 - 2.2 0.1 0.0 - 0.09 0.02 0.0 - 0.2 0.2 0.03 ns 0.037
CCR9 1.0 0.5 - 1.3 0.2 0.1 - 0.8 0.2 0.1 - 0.3 0.2 0.2 0.01 0.007
Cytokines
IFN-γ 1.7 0.9 - 8.7 10.6 3.4 - 17.0 11.0 4.4 - 17.0 6.2 6.5 0.008 0.02
IL-10 1.2 0.8 - 4.5 2.1 1.1 - 3.7 2.3 1.7 - 3.7 1.8 1.9 ns 0.046
TNFα 1.0 0.4 - 1.4 0.6 0.03 - 1.1 0.3 0.03 - 1.1 0.6 0.3 ns ns
IFN-α2 NDe ND ND ND ND ND ND
IFN-β 1.1 0.2 - 3.7 1.4 0.4 - 2.9 1.4 0.4 - 2.9 1.3 1.3 ns ns
TGF-β1 1.3 1.0 - 1.7 1.0 0.2-1.4 0.9 0.2-1.1 0.8 0.7 0.025 0.011
Other Immune-relevant Markers
TLR3 1.9 1.0 - 4.5 3.5 2.1 - 11.8 3.8 2.1 - 6.5 1.8 2.0 0.037 ns
DC-SIGN 0.7 0.4 - 1.1 0.2 0.1 - 0.4 0.2 0.1 - 0.4 0.3 0.3 0.001 0.004
FOXP3 0.9 0.7 - 2.0 0.3 0.1 - 0.9 0.3 0.1 - 0.3 0.3 0.3 0.004 0.005
RORγt 1.2 0.5 - 2.9 0.2 0.1 - 1.0 0.1 0.1 - 0.2 0.2 0.1 0.011 0.016
a

Inclusion criteria for this table were either a greater than 2-fold increase after SIV infection or a p-value <0.05 from a Mann-Whitney nonparametric test (all SIV infected animals versus uninfected controls, or AIDS-developing animals versus uninfected controls). The mRNAs encoding chemokines CCL2, CCL19, CX3CL1, CXCL13, XCL1, CCR1, CCR3, CCR11, CXCR1, CXCR4, CXCR5 and CX3CR1 were also measured but did not change in expression after SIV infection according to these criteria.

b

The median values provided are for the indicated group (UI, uninfected and two macaques determined to be exposed but uninfected [n=6]; all SIV infected animals, including two acutely infected, two chronically infected, and six AIDS-developing macaques [n=10]; or all animals developing AIDS [n=6]), with the individual animal’s expression values normalized to the β-GUS endogenous control and then normalized to the same uninfected control “calibrator” sample.

c

Not significant (P>0.05).

d

Chemokine receptor mRNAs for CCR2, CCR10, CXCR2 and XCR1 mRNAs were not detected.

e

ND, not detected.