Table 2.
Phosphopeptide sequences obtained from TiO2-LC-MS/MS analysis. All phosphorylation sites are non-localized except the one contains Ser 287. Residue numbers were based on the OPN isoform NP_001191131. The bottom row contains the total number of the spectral counts of individual phosphopeptides in each genotype.
| phosphopeptide sequence | Bone ColTg | Bone WT | Bone ColTg; Alpl−/− | Bone Alpl−/− | Ostb WT | Ostb ColTg; Alpl−/− | Ostb Alpl−/− |
|---|---|---|---|---|---|---|---|
| Vp(T38DS40GS42S43EEKLYS49)LHPDPIATWL | 21 | 0 | 7 | 1 | 0 | 0 | 0 |
| p(S174FQVS178DEQY182PDAT186DEDLT191)SHMK | 0 | 53 | 91 | 84 | 0 | 0 | 18 |
| p(S192HMKS196GES199KES202)LDVIPVAQLL | 7 | 11 | 13 | 36 | 3 | 0 | 2 |
| LEHp(S244KES247QES250)ADQSDVIDSQASSK | 0 | 8 | 7 | 31 | 0 | 0 | 4 |
| LVLDPK(pS287)KEDDR | 29 | 39 | 59 | 31 | 7 | 9 | 38 |
| FRIpp(S299HELES304S305S306S307)EVN | 25 | 22 | 24 | 21 | 1 | 0 | 4 |
| FRIp(S299HELES304S305S306S307)EVN | 51 | 36 | 65 | 63 | 8 | 10 | 38 |
| 133 | 169 | 266 | 267 | 19 | 19 | 100 |