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. 2013 Jun 20;8(6):e66159. doi: 10.1371/journal.pone.0066159

Figure 6. Transporter gene expression in human and rat podocytes.

Figure 6

Results from RT-PCR and QRT-PCR analysis of membrane transporters in human cell lines (a,b) and primary rat podocytes (c,d). (a) RT-PCR analysis of human cells lines; P  =  human podocytes, N SHSY-5Y =  neuroblastoma cells; I Caco2  =  colon cancer cells. (b) QRT-PCR analysis of membrane transporters using the same primers in human podocytes. (c) RT-PCR analysis of primary rat podocytes; the results compare gene expression of cells obtained from three distinct isolation procedures (I, II, III). (d) QRT-PCR analysis of Oatps members in primary rat podocytes. Results are expressed as mean ± SEM of three independent experiments. NTC  =  RT-PCR negative control.