Table 2. Investigated human transporters.
Type PCR | GBN | Protein and Gene Symbol | Primer pair | TA (°C)a | Amplif. region | Length(bp) |
RT-PCR/QRT-PCR | NM 134431 | OATP1A2, (SLCO1A2) OATP-A | (F) 5’-CCATTGGAACGGGAATAAACA-3’ (R) 5’-TCTCTTCAGATTTCATACACCTCA -3’ | 60° | 2136–2351 | 216 |
NM 007256 | OATP2B1, (SLCO2B1) OATP-B | (F) 5’-GGACAACAGCCAGGTTTTCTACAC-3’ (R) 5’-AGGAAGGGCACCACCAGATG-3’ | 60° | 1851–1958 | 108 | |
NM 006446 | OATP1B1, (SLCO1B1) OATP-C | (F) 5’-TAAGGCTAACATCTTATTGGGAGTC-3’(R) 5’-ACAGCAGTAAAACATGAGAATTTGG-3’ | 60° | 1250–1382 | 133 | |
NM 013272 | OATP3A1, (SLCO3A1) OATP-D | (F) 5’-AGCCCTGGACCCCTACTCG-3’(R) 5’- CCCGTGAGATTCGTGCTGTTG-3’ | 60° | 1582–1716 | 135 | |
NM 016354 | OATP4A1, (SCL21A12) OATP-E | (F) 5’-CACCAGTTGAAGGACAGCAG-3’(R) 5’-AGGAGCCAGATGGAGAGAGG-3’ | 60° | 1253–1341 | 89 | |
NM 004254 | OAT3 (SLC22A8) | (F) 5’-CCCACAGTCATCAGGCAAACA-3’(R) 5’-AGGGCGGTGATCCCGTAGA-3’ | 60° | 1448–1584 | 137 | |
NM 000927 | MDR1 (ABCB1) | (F) 5’- TGCTCAGACAGGATGTGAGTTG-3’(R) 5’-AATTACAGCAAGCCTGGAACC-3’ | 60° | 2808-2926 | 122 | |
NM 005073 | PEPT1 (SLC15A1) | (F) 5’- CACCTCCTTGAAGAAGATGGCA-3’(R) 5’- GGGAAGACTGGAAGAGTTTTATCG-3’ | 60° | 1121-1225 | 105 | |
NM 021082 | PEPT2 (SLC15A2) | (F) 5’-TTTGTCTCTCTACACTGAGCATTC-3′(R) 5′ -CTGCTTTCTGTATCCTTTACCATC-3′ | 60° | 1453-1563 | 111 | |
NM 002046 | GAPDH | (F) 5′-CCCATCACCATCTTCCAGGAG-3′(R) 5′- CTTCTCCATGGTGGTGAAGACG-3′ | 60° | 319-423 | 105 | |
Only RT-PCR | NM 005073 | PEPT1 (SLC15A1) | (F) 5′-TCAGGTCGGAGGAGTAGC-3′(R) 5′ -AGAATTGGCGTCAGGTAGC-3′ | 55° | 4-283 | 279 |
NM 021082 | PEPT2 (SLC15A2) | (F) 5′- GAAGCCATCTCCGACAATCTG-3′(R) 5′ -GCCAAGCACATACACCAAGG -3′ | 60° | 245- 525 | 280 |
GBN Gene Barnk Number; TA Annealing Temperature.