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. 2013 Jun 20;8(6):e66159. doi: 10.1371/journal.pone.0066159

Table 2. Investigated human transporters.

Type PCR GBN Protein and Gene Symbol Primer pair TA (°C)a Amplif. region Length(bp)
RT-PCR/QRT-PCR NM 134431 OATP1A2, (SLCO1A2) OATP-A (F) 5’-CCATTGGAACGGGAATAAACA-3’ (R) 5’-TCTCTTCAGATTTCATACACCTCA -3’ 60° 2136–2351 216
NM 007256 OATP2B1, (SLCO2B1) OATP-B (F) 5’-GGACAACAGCCAGGTTTTCTACAC-3’ (R) 5’-AGGAAGGGCACCACCAGATG-3’ 60° 1851–1958 108
NM 006446 OATP1B1, (SLCO1B1) OATP-C (F) 5’-TAAGGCTAACATCTTATTGGGAGTC-3’(R) 5’-ACAGCAGTAAAACATGAGAATTTGG-3’ 60° 1250–1382 133
NM 013272 OATP3A1, (SLCO3A1) OATP-D (F) 5’-AGCCCTGGACCCCTACTCG-3’(R) 5’- CCCGTGAGATTCGTGCTGTTG-3’ 60° 1582–1716 135
NM 016354 OATP4A1, (SCL21A12) OATP-E (F) 5’-CACCAGTTGAAGGACAGCAG-3’(R) 5’-AGGAGCCAGATGGAGAGAGG-3’ 60° 1253–1341 89
NM 004254 OAT3 (SLC22A8) (F) 5’-CCCACAGTCATCAGGCAAACA-3’(R) 5’-AGGGCGGTGATCCCGTAGA-3’ 60° 1448–1584 137
NM 000927 MDR1 (ABCB1) (F) 5’- TGCTCAGACAGGATGTGAGTTG-3’(R) 5’-AATTACAGCAAGCCTGGAACC-3’ 60° 2808-2926 122
NM 005073 PEPT1 (SLC15A1) (F) 5’- CACCTCCTTGAAGAAGATGGCA-3’(R) 5’- GGGAAGACTGGAAGAGTTTTATCG-3’ 60° 1121-1225 105
NM 021082 PEPT2 (SLC15A2) (F) 5’-TTTGTCTCTCTACACTGAGCATTC-3′(R) 5′ -CTGCTTTCTGTATCCTTTACCATC-3′ 60° 1453-1563 111
NM 002046 GAPDH (F) 5′-CCCATCACCATCTTCCAGGAG-3′(R) 5′- CTTCTCCATGGTGGTGAAGACG-3′ 60° 319-423 105
Only RT-PCR NM 005073 PEPT1 (SLC15A1) (F) 5′-TCAGGTCGGAGGAGTAGC-3′(R) 5′ -AGAATTGGCGTCAGGTAGC-3′ 55° 4-283 279
NM 021082 PEPT2 (SLC15A2) (F) 5′- GAAGCCATCTCCGACAATCTG-3′(R) 5′ -GCCAAGCACATACACCAAGG -3′ 60° 245- 525 280

GBN Gene Barnk Number; TA Annealing Temperature.