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. 2013 Jun 20;8(6):e64812. doi: 10.1371/journal.pone.0064812

Figure 2. TKO mice produce increased proinflammatory cytokines.

Figure 2

(A) TNF-α level in the WT and TKO mouse serum were measured by Ready-set-go ELISA kits (eBiosciences). Data are shown as means±SD, n = 9, p = 0.0002. (B) Thioglycollate-induced peritoneal macrophages (MФ) were treated with LPS for 2, 3, 4 and 5 hrs. TNF-α released into medium was measured as above. Data are shown as means ±SD for five wells per group in a single experiment and are representative of those in three experiments. N = 3, **P<0.001. (C) Real-time qPCR quantification of IL-1β and IL-6 mRNA in the lymph nodes. The total RNA was extracted by TRIzol and reversely transcribed using transcribed using qScript™ cDNA Supermix kit (Quanta Biosciences, MD). Real time qPCR was performed to measure the relative mRNA level of IL-1β and IL-6 genes in the WT (open) and TKO (solid) L.N. Data are shown as means ± SD, n = 3, *P<0.05, **P<0.002. Statistics was performed by the one-way ANOVA test using ProStat Ver 5.5.