Figure 1.

Cleavage products of C3 deposit on PP mesh after plasma-biomaterial interaction. Lepirudin-anticoagulated plasma (100 μl) isolated from healthy donors was incubated with mesh (5×5 mm) for 60 min, and deposition of C3 cleavage products was measured by ELISA. Treatment of plasma with the compstatin analog Cp40 (20 μM) before mesh addition abolished complement deposition when compared to plasma samples treated with an inactive control peptide (20 μM; n=5). Incubation of plasma alone or incubation of plasma with mesh in the presence of EDTA (10 mM) served as negative control. *P < 0.05; 1-way ANOVA.