Figure 4. Effect of overexpression of HIFs on the expression of ETS-family transcription factors.
(A) Western blot analysis of HIF-1α, HIF-2α, and ETS-family transcription factors after overexpression of HIF-1α or HIF-2α using human embryonic kidney (HEK) 293 cells. β-actin is an internal control. (B) Quantitavive real-time reverse-transcription PCR (qRT-PCR) of ETS-family transcription factors after overexpression of HIF-1α or HIF-2α using HEK293 cells. Data are shown as relative to the expression of β-actin.