Table 4. Metabolite concentrations (µmol/gCDW) in the wild-type E. coli Nissle 1917, ΔcsrBC and ΔcsrA51 mutants.
Glucose | Gluconate | |||||
Metabolite | wild-type | ΔcsrBC | ΔcsrA51 | wild-type | ΔcsrBC | ΔcsrA51 |
1,3-diPGa | 2.28±1.06 | 3.89±3.76 | 1.90±0.15 | 4.45±0.56 | 3.30±3.00 | 3.68±0.73 |
2/3-PG | 1.42±0.11 | 1.67±0.52 | 1.12±0.23 | 4.20±0.33 | 4.66±0.45 | 2.66±0.20 |
6PG | 0.38±0.09 | 0.38±0.07 | 0.77±0.20 | 3.59±0.21 | 2.33±0.55 | 0.65±0.79 |
ADP | 0.71±0.06 | 0.82±0.25 | 0.91±0.27 | 0.95±0.28 | 1.01±0.35 | 0.80±0.19 |
AMP | 0.16±0.03 | 0.11±0.09 | 0.21±0.06 | 0.20±0.05 | 0.40±0.10 | 0.17±0.02 |
ATP | 3.50±0.29 | 3.21±0.03 | 2.86±0.48 | 3.51±0.15 | 4.08±0.15 | 2.95±0.24 |
cAMP | 0.01±0.02 | 0.00±0.02 | 0.01±0.01 | 0.01±0.01 | 0.01±0.01 | 0.01±0.03 |
Cit | 1.01±0.90 | 0.81±0.33 | 0.40±0.14 | 0.27±0.10 | 0.35±0.25 | 0.57±1.05 |
F1P | 0.03±0.01 | 0.04±0.01 | 0.02±0.01 | 0.19±0.11 | 0.21±0.05 | 0.14±0.05 |
F6P | 0.44±0.03 | 0.41±0.03 | 0.90±0.08 | 0.26±0.02 | 0.19±0.03 | 0.19±0.01 |
FBP | 2.01±0.55 | 2.30±0.12 | 1.44±0.32 | 2.95±0.47 | 2.51±0.14 | 1.82±0.09 |
Fum | 0.59±0.34 | 0.56±0.16 | 0.44±0.09 | 0.75±0.26 | 0.72±0.10 | 0.63±0.15 |
G6P | 1.78±0.19 | 1.93±0.61 | 2.00±0.40 | 0.97±0.70 | 0.48±0.05 | 0.39±0.05 |
M6P | 0.26±0.04 | 0.26±0.04 | 0.56±0.17 | 0.05±0.03 | 0.12±0.01 | 0.15±0.01 |
Mal | 1.82±0.45 | 0.93±0.30 | 0.95±0.30 | 1.64±0.57 | 2.29±0.29 | 2.35±0.67 |
PEP | 0.19±0.05 | 0.21±0.08 | 0.21±0.05 | 0.49±0.05 | 0.54±0.12 | 0.49±0.11 |
R1P | 0.02±0.01 | 0.01±0.001 | 0.01±0.01 | 7.81±0.81 | 42.2±9.2 | 17.9±4.3 |
R5P | 0.75±0.06 | 0.73±0.28 | 0.71±0.15 | 1.93±0.45 | 2.60±0.16 | 3.38±0.41 |
S7Pa | 4.63±0.31 | 4.17±1.19 | 12.8±1.5 | 19.9±1.5 | 27.8±2.5 | 52.9±2.7 |
Suc | 4.44±0.27 | 5.34±1.52 | 1.98±0.46 | 3.34±0.38 | 5.58±0.85 | 6.69±0.56 |
UDP-Glucose | 4.6 5±0.14 | 4.86±0.47 | 5.20±0.47 | 2.86±0.31 | 3.79±0.57 | 2.14±0.56 |
Values represent the mean ± standard deviation of three independent biological replicates.
For these metabolites only the 12C/13C ratio is given. This ratio represents the concentration of the metabolite in samples relative to the IDMS standard, which added at the same amounts in all samples.