Figure 8. Effects of CrataBL and SbTI on the viability of the DU145 (A, C) and PC3 (B, D) cell lines, respectively.

Cells were incubated in 96-well microplates in the concentration of 5×10³ cells/100 µL/well. After 24 h, CrataBL, in the final concentrations of 5, 10, 20 and 40 µM, was incubated at 37°C in an atmosphere of 5% (v/v) CO₂ for 24, 48 and 72 h. The viability was determined by MTT colorimetric method. Results represent the mean ± standard deviation of three experiments, each conducted in triplicate (*p<0.05; ** p<0.01; *** p<0.001 compared with control cells). In the absence of CrataBL, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction was considered as 100%.