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. 2013 Jun 18;8(6):e64426. doi: 10.1371/journal.pone.0064426

Figure 9. CrataBL induced apoptosis in prostate cancer cells.

Figure 9

The cell lines were seeded in 6-well plates for 24 h, followed by washing three times and incubating with RPMI without FBS for cell cycle synchronization. Afterwards cells were treated with CrataBL (40 µM) or medium (control) for 24 h (A and B) and 48 h (C and D). The analysis was performed in FACSCalibur flow cytometer using annexin V-FITC and propidium iodide (PI) staining. The percentage of viable (An−PI−), apoptotic (An+PI−), secondary apoptotic (An+PI+) and necrotic (An−PI+) cells are represented. Data expressed as mean ± SD, obtained from experiments performed in triplicate. (*p<0.05; *** p<0.001 compared with control cells).