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. 2013 Jun 18;8(6):e65535. doi: 10.1371/journal.pone.0065535

Figure 2. Generation of CsDFR and CsANR transgenic tobacco.

Figure 2

A, Graphic representation of pCAMBIA 1302 vector with cDNA of CsDFR and CsANR. CsDFR and CsANR cDNA was inserted in-between the NcoI and BglII restriction site of pCAMBIA 1302. B, Genomic DNA PCR confirmed the insertion of CsDFR and CsANR cDNA in plant genome of transgenic lines. C, Semi-quantitative PCR documented the transcript expression levels of CsDFR and CsANR in transgenic tobacco lines. Housekeeping gene 26S rRNA was used as internal control for expression study and experiments were repeated at least three times with similar results.