Figure 5. ChIP-qPCR demonstrating the relative quantity of p_fim bound by FimR.

Cells were grown under 0.01 µM MnCl₂ and 0.1 µM FeSO₄ (I), 0.01 µM MnCl₂ and 50 µM FeSO₄ (II), 50 µM MnCl₂ and 0.1 µM FeSO₄ (III), and 50 µM MnCl₂ and 50 µM FeSO₄ (IV). The ΔCq of the sample from III was used as the reference. Significant differences between samples were determined using one-way ANOVA. A significant difference (P<0.05) was detected between all pairs of comparison.