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. 2013 Jun 18;8(6):e66163. doi: 10.1371/journal.pone.0066163

Table 2. Primers used in this study.

Primer Sequencea
fimC/ASBamHI GAATCATGGATCCGCTCCTTTACTATT
fimC/AS5025 GGATGGTTGGACCCTGGATGGTG
fimC_5′/S4731 GCTGTCTCAGCCCATTCCCCGTTGACG
fimR/AS9576SmaI AAACCCGGGCTTCTTTGTTTGGTGTCATG
fimR/AS10331PstI GAAGTCCCTGCAGAACGAGGGATCTTT
fimR/AS10915SacI GCCGGAGCTCTACTCTGTTAAGCGTAC
fimR/S8023SpeI AGGACTAGTACCTCTTTTCTATATCTAC
fimR/S9221BamHI CATGAGGATCCTCAACAAGTCTTGGGC
fimR/S9761SmaI AAACCCGGGTCTCTGATCTCTACCG
fimR/MSSacI GAAGAAAAGAGGAGCTCATGACACC
fimR/stopASPstI GAAAGCATGCACTGCAGTTAGACTGCA
pDS-1 ATCAAGTCCATAAAACCAC
pDS-2 CATCTTGTAAACGTACCATGATC
pDS-3 CTAATAACCTTATCAAAAATAGGAAC
pDS-4 TAATCTAAATTATTTTATAAATTAACTTG
pDS-5 TTGACTTAATTTTTTTATTTATGTATATTA
pepO/AS320SacI GTACCGAGCTCGCTGGTATAGTCTT
pfimbox/AS TTACTATTAATATACATAAATAAAAAAATTAAGTCAAGTTAATTTATAAAATA
pfimbox/S TATTTTATAAATTAACTTGACTTAATTTTTTTATTTATGTATATTAATAGTAA
spe/AS GCAACTGCAGATTGTTTTCTAAAATCTGATT
tcrB/AS CTATTTCTAAGGCTTGGCGG
tcrB/S TGGCGATGAAGTAATCGGGG
a

inserted restriction recognition sites are underlined.