Table 2. Primers used in this study.
Primer | Sequencea |
fimC/ASBamHI | GAATCATGGATCCGCTCCTTTACTATT |
fimC/AS5025 | GGATGGTTGGACCCTGGATGGTG |
fimC_5′/S4731 | GCTGTCTCAGCCCATTCCCCGTTGACG |
fimR/AS9576SmaI | AAACCCGGGCTTCTTTGTTTGGTGTCATG |
fimR/AS10331PstI | GAAGTCCCTGCAGAACGAGGGATCTTT |
fimR/AS10915SacI | GCCGGAGCTCTACTCTGTTAAGCGTAC |
fimR/S8023SpeI | AGGACTAGTACCTCTTTTCTATATCTAC |
fimR/S9221BamHI | CATGAGGATCCTCAACAAGTCTTGGGC |
fimR/S9761SmaI | AAACCCGGGTCTCTGATCTCTACCG |
fimR/MSSacI | GAAGAAAAGAGGAGCTCATGACACC |
fimR/stopASPstI | GAAAGCATGCACTGCAGTTAGACTGCA |
pDS-1 | ATCAAGTCCATAAAACCAC |
pDS-2 | CATCTTGTAAACGTACCATGATC |
pDS-3 | CTAATAACCTTATCAAAAATAGGAAC |
pDS-4 | TAATCTAAATTATTTTATAAATTAACTTG |
pDS-5 | TTGACTTAATTTTTTTATTTATGTATATTA |
pepO/AS320SacI | GTACCGAGCTCGCTGGTATAGTCTT |
pfimbox/AS | TTACTATTAATATACATAAATAAAAAAATTAAGTCAAGTTAATTTATAAAATA |
pfimbox/S | TATTTTATAAATTAACTTGACTTAATTTTTTTATTTATGTATATTAATAGTAA |
spe/AS | GCAACTGCAGATTGTTTTCTAAAATCTGATT |
tcrB/AS | CTATTTCTAAGGCTTGGCGG |
tcrB/S | TGGCGATGAAGTAATCGGGG |
inserted restriction recognition sites are underlined.