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. 2013 Jun 18;8(6):e65950. doi: 10.1371/journal.pone.0065950

Figure 4. V3 loop alterations important for CXCR4 usage by C-HIV harbored by subject 1109.

Figure 4

Luciferase reporter viruses pseudotyped with Env mutants (M1 through M15), unmodified 1109-E-10 and 1109-F-30 Envs, or control Envs (ADA, HXB2, 89.6) were used to infect NP2-CD4/CXCR4 and NP2-CD4/CCR5 cells, and the levels of HIV-1 entry were determined as described in Materials and Methods. Open bars indicate Envs with an R5 phenotype, black bars indicate Envs with an X4 phenotype, and hatched bars indicate Envs with an R5X4 phenotype. The dotted lines indicate the limit of detection of coreceptor activity. The results shown are a compilation of 3 independent experiments, each performed in triplicate, and each with an independent preparation of Env-pseudotyped virus. The data shown are means, and the error bars represent standard errors of the means.