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. 2013 Jun 18;8(6):e66276. doi: 10.1371/journal.pone.0066276

Figure 2. Analysis of mAbs against HAs of clade 0, 1, and 2 H5N1 viruses.

Figure 2

(A) Amino acid sequences of VH and VL region and their closest corresponding human Ig gene. (B) Immunofluorescent analysis of mAbs. The MDCK cells were infected with H1N1 influenza virus A/Puerto Rico/8/34 (PR8) or recombinant influenza viruses carrying HA from clade 0, 1, and 2 H5N1 viruses in r97HKHA-PR8, r04VNHA-PR8, and r06ZJHA-PR8. (C) Western blot analysis. The HA proteins expressed in MDCK cells infected with r97HKHA-PR8, r04VNHA-PR8, r06ZJHA-PR8 or A/Puerto Rico/8/34 were used to determine the binding of the mAbs. Arrows indicate the specific bands for the corresponding proteins.