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. 2013 Jun 18;8(6):e66364. doi: 10.1371/journal.pone.0066364

Figure 8. Expression analysis of Fugu cytokine genes in CD4+ cells sorted from PBLs under immunostimulatory conditions.

Figure 8

RNA isolated from CD4+ cells incubated with 20 µg/ml LPS, 20 µg/ml polyI:C or 20 µg/ml ConA for 0 (cont.), 6, 12 and 24 h. Fugu cytokine mRNA transcripts were determined by multiplex RT-PCR and standardized to the respective β-actin mRNA. The analysed genes were as follows: 1) IL-2, 2) IFN-γ, 3) TNF-α, 4) IL-4/13A, 5) IL-4/13B, 6) IL-17A/F-3, 7) TGF-β1, and 8) IL-10. Data are presented as mean ± S.D. of triplicate samples. *P<0.01 as compared to the control (upregulation). The relative expression level is expressed as arbitrary units where one unit is equal to the average expression level of each cytokine gene in CD4+ cells from unstimulated PBLs. Gray, Blue, red and green bars indicate HBSS, LPS, polyI:C and ConA-stimulations, respectively.