Skip to main content
. 2013 Jun 18;8(6):e66364. doi: 10.1371/journal.pone.0066364

Table 1. Primers designed for expression analysis of marker genes in this study.

Name Sequence (5′–3′) Length (mer)
CSF1R1 Fw TTTACCGACACCGCGGGATT 20
CSF1R1 Rv GCCGCTGTCACTTCTAATGTA 20
CSF1R2 Fw GGCATGAACGTGACTGTTGA 20
CSF1R2 Rv CATGGTACCCAAGGTGACTT 20
CD4 Fw AAGCCTCAGAGGGAACAGAA 20
CD4 Rv GAAGAACGTGGTCGATACGA 20
CD4REL Fw GGAAGCAGATGCAGGAATGT 20
CD4REL Rv GCCTGTGACTCTGATATCCA 20
CD8α Fw CCCAGGTGGACATTCATTGT 20
CD8α Rv TTGTTGCCTCGGCGTCGTTT 20
TCRα Fw AGCGCATGTCTGGCCACAGGTTTCA 25
TCRα Rv GACTGATACGCAGACGAAGAGTCATCAGG 29
TCRβ Fw TCCTCCAGAGAGTGTCGCAA 20
TCRβ Rv TGCAGCTTCCAGGCCAGAAA 20
TCRγ Fw CAGGGCTGGTTGTCATAGAA 20
TCRγ Rv GCATCTGAGACGACGAGTCT 20
TCRδ Fw TCAGCAGCAGAGAAGGAGAA 20
TCRδ Rv ACTCAGGACTGTCGGATCTT 20
CD3ε Fw CCAACCGATTAGAGCGAATCAGAGGC 26
CD3ε Rv CCTGATTCCTTCCCGGATCCAGC 23
CD28 Fw GAAGGTAGAAGGGAAGACAAGCGTCCC 27
CD28 Rv CTTTCACATAGCAGGACAGGGCGAC 25
CD154 Fw CTGGGACTGGAAACATGGTCACTCTCGG 28
CD154 Rv GCAACACTGTACAGGGTTCCATCCGCGG 28
IgL Fw GAAGGTAGAAGGGAAGACAAGCGTCCC 27
IgL Rv CTTTCACATAGCAGGACAGGGCGAC 25
IgM Fw CTGGGACTGGAAACATGGTCACTCTCGG 28
IgM Rv GCAACACTGTACAGGGTTCCATCCGCGG 28
T-bet Fw CCGTGACAATTACGACACGC 20
T-bet Rv GAGAGGTAGCCTTGGGGGTA 20
GATA-3 Fw CAACCGACCTCTGACCATGA 20
GATA-3 Rv CCATGCTGTCCTGGGACTTT 20
FoxP3 Fw GTGGAAGGTCCAACAGGACA 20
FoxP3 Rv CGGAATCTGACTGCTGGTCT 20