Table 1. Primers designed for expression analysis of marker genes in this study.
Name | Sequence (5′–3′) | Length (mer) |
CSF1R1 Fw | TTTACCGACACCGCGGGATT | 20 |
CSF1R1 Rv | GCCGCTGTCACTTCTAATGTA | 20 |
CSF1R2 Fw | GGCATGAACGTGACTGTTGA | 20 |
CSF1R2 Rv | CATGGTACCCAAGGTGACTT | 20 |
CD4 Fw | AAGCCTCAGAGGGAACAGAA | 20 |
CD4 Rv | GAAGAACGTGGTCGATACGA | 20 |
CD4REL Fw | GGAAGCAGATGCAGGAATGT | 20 |
CD4REL Rv | GCCTGTGACTCTGATATCCA | 20 |
CD8α Fw | CCCAGGTGGACATTCATTGT | 20 |
CD8α Rv | TTGTTGCCTCGGCGTCGTTT | 20 |
TCRα Fw | AGCGCATGTCTGGCCACAGGTTTCA | 25 |
TCRα Rv | GACTGATACGCAGACGAAGAGTCATCAGG | 29 |
TCRβ Fw | TCCTCCAGAGAGTGTCGCAA | 20 |
TCRβ Rv | TGCAGCTTCCAGGCCAGAAA | 20 |
TCRγ Fw | CAGGGCTGGTTGTCATAGAA | 20 |
TCRγ Rv | GCATCTGAGACGACGAGTCT | 20 |
TCRδ Fw | TCAGCAGCAGAGAAGGAGAA | 20 |
TCRδ Rv | ACTCAGGACTGTCGGATCTT | 20 |
CD3ε Fw | CCAACCGATTAGAGCGAATCAGAGGC | 26 |
CD3ε Rv | CCTGATTCCTTCCCGGATCCAGC | 23 |
CD28 Fw | GAAGGTAGAAGGGAAGACAAGCGTCCC | 27 |
CD28 Rv | CTTTCACATAGCAGGACAGGGCGAC | 25 |
CD154 Fw | CTGGGACTGGAAACATGGTCACTCTCGG | 28 |
CD154 Rv | GCAACACTGTACAGGGTTCCATCCGCGG | 28 |
IgL Fw | GAAGGTAGAAGGGAAGACAAGCGTCCC | 27 |
IgL Rv | CTTTCACATAGCAGGACAGGGCGAC | 25 |
IgM Fw | CTGGGACTGGAAACATGGTCACTCTCGG | 28 |
IgM Rv | GCAACACTGTACAGGGTTCCATCCGCGG | 28 |
T-bet Fw | CCGTGACAATTACGACACGC | 20 |
T-bet Rv | GAGAGGTAGCCTTGGGGGTA | 20 |
GATA-3 Fw | CAACCGACCTCTGACCATGA | 20 |
GATA-3 Rv | CCATGCTGTCCTGGGACTTT | 20 |
FoxP3 Fw | GTGGAAGGTCCAACAGGACA | 20 |
FoxP3 Rv | CGGAATCTGACTGCTGGTCT | 20 |