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. 2013 Jun 18;8(6):e66306. doi: 10.1371/journal.pone.0066306

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© 2013 Zhou et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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ARPE-19 cells were transfected with Nox4-specific siRNA for 48 h. (A) Nox4 knockdown efficiency was determined by western blot. (B) Intracellular ROS levels were measured by flow cytometry after DHE staining. (C) ARPE-19 cells transfected with control siRNA or specific Nox4 siRNAs were infected with T. gondii for 24 h, and fluorescence micrographs of intracellular tachyzoite replication 24 h post-infection were obtained. The results are expressed as mean ± SD of three independent experiments.