Figure 10. Increased proteolysis of PARP in MPTQ treated neuro 2a neuroblastoma cells.

A) Neuro 2a cells were cultured and treated with 30 µM of MPTQ for 24 hours and lysates were prepared from three independent treatments. 60 µg of total protein were size fractionated in 12% SDS-polyacrylamide gel and immunoblotted with anti-PARP antibody that detects both full length PARP and cleaved PARP. Cleaved PARP is seen only in MPTQ treated N2a cells but not at all in control cells indicating the proteolysis of PARP, a hallmark feature in apoptotic cells. B) Immunocytochemistry of PARP using an antibody specific for cleaved PARP. Detection was done using Alexa fluor 594 labelled secondary antibodies. Nuclei were stained with DAPI. Nuclei with cleaved PARP were seen only in MPTQ treated cells. The figure represents at least three independent experiments.