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. 2013 Jun 18;8(6):e66471. doi: 10.1371/journal.pone.0066471

Figure 4. Pervanadate and Dasatinib do not affect resting or caerulein induced changes in cytosolic calcium.

Figure 4

Cytosolic calcium levels in Fura-2AM loaded acini measured over 10 minutes. Arrow indicates time of addition of the stimulus. 100 µM pervanadate (PV, blue diamonds) does not result in an increase in resting cytosolic calcium levels, which remain unchanged in its presence, similar to basal acini (BASAL, black triangles). 100 nM Caerulein (100 nM CER, red squares), causes a prompt increase in cytosolic calcium levels, which is not reduced in the presence of 10 µM Dasatinib (100 nM CER+10 µM DAS, pink squares). Each data point represents mean value calculated over multiple (n≥3) experiments, in each of which 7–25 acini per field were analyzed. Standard error of mean is depicted as a bar. Asterisks correspond to time points at which calcium levels were significantly different (p<0.05) from basal levels compared to either conditions with 100 nM Caerulein alone, or to the same in the presence of 10 µM Dasatinib. Calcium levels in conditions with 100 µM Pervanadate were not significantly different from basal.