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Seven days after the last immunization, serum was isolated and assayed for IL-4, IL-10, IL-12 and IFN-γ production in response to rSjMF or ISA 206 adjuvant alone, as a control. Significantly increased serum cytokine concentration compared with the ISA 206 adjuvant control is denoted by **P<0.01 or ***P<0.001.
