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. 2013 Jun 18;8(6):e66561. doi: 10.1371/journal.pone.0066561

Figure 3. Regulatory effect of gliadin peptide 10-mer on COX-2 expression and activity in PT-Gly-stimulated CACO-2/TC7 cells.

Figure 3

(A) CACO-2/TC7 cells, either unstimulated or stimulated with PT-Gly (1mg/ml), p10-mer (50 µg/ml), p10-mer (50 µg/ml) + PT-Gly (1mg/ml), were analyzed by Western blot for COX-2 expression. COX-2 expression was analyzed in whole cell extracts by Western blot with anti-COX-2 antibodies; for control, the blotted membranes were stripped and reprobed with anti-α tubulin antibodies. Bound antibodies were visualized with HRP-conjugated IgG and immunoreactivity was assessed by ECL. Densitometric analysis was performed using ImageJ version 1.46 software, peaks were reproduced by reading the Western Blot bands. (B) CACO-2/TC7 cells, either unstimulated or stimulated with PT-Gly (1mg/ml), p10-mer (50 µg/ml), p10-mer (50 µg/ml) + PT-Gly (1mg/ml) were analyzed for COX-2 enzymatic activity by measuring PGE-2 release with PGE-2 assay kit. Statistical analysis: *P<0.01 versus control; §P<0.05 versus PT-Gly. Results are expressed as mean ± SD; n = 3.