Figure 1. Effects of UvsX protein on fluorescence emission spectra of AlexaFluor 546-labeled oligonucleotides.

Conditions were as described in Materials and Methods. The fluorescence emission spectra of (A) 2 µM (nucleotide pairs) double-stranded oligonucleotide dT₂₅:dA₂₂XA₂ (oligo 2:oligo 1), and (B) 2 µM (nucleotides) single-stranded oligonucleotide dA₂₂XA₂ (oligo 1), were recorded in the absence of UvsX (solid line), in the presence of a saturating amount of UvsX (2 µM) (dashed line), and in the presence of saturating amounts of both UvsX and ATP (2.5 mM) (dotted line).