Figure 4. Binding of UvsX to ssDNA in the presence of increasing amounts of homologous dsDNA 3 µM UvsX (final concentration) was added to mixtures containing final concentrations of 2 µM ssDNA (dA₂₂XA₂, oligo 1), 2.5 mM ATP, and various concentrations of homologous dsDNA (dT₂₅:dA₂₅, oligo 2:oligo 4).

The amplitude of fluorescence quenching was graphed as a function dsDNA and a line was fit (solid line) to demonstrate the trend.