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. 2013 Jun 18;8(6):e66654. doi: 10.1371/journal.pone.0066654

Figure 6. Binding of UvsX to dsDNA in the presence of excess homologous or heterologous ssDNA.

Figure 6

Various amounts of UvsX were added to mixtures containing final concentrations of 2 µM dsDNA (dT25:dA22XA2 oligo 2: oligo 1), 2.5 mM ATP, plus 40 µM of either homologous (dA25, oligo 4) or heterologous (dC25 oligo 3) ssDNA. The amplitude of fluorescence quenching was graphed as a function of UvsX concentration. Data for homologous ssDNA were fit to Equation 1 to determine an apparent Kd value (black line). Data for heterologous ssDNA were fit to a line (gray line).