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. 2013 Jun 18;8(6):e66712. doi: 10.1371/journal.pone.0066712

Figure 2. Detailed dynamics of short RecA filaments in different KCl concentrations.

Figure 2

(A–B) Time traces of polymerization and de-polymerization of RecA nucleoprotein filament on a 595 bp dsDNA in 1 µM RecA 1 mM ATP, 1x ATP regeneration system, 24°C, pH 7.4, with 50 mM KCl (A) first then 150 mM KCl (B) next. Data in the left panels were recorded at different forces indicated by different colors. Right panels show dynamics of the competition between polymerization and de-polymerization under a constant force of ∼34.1 pN. Inset shows the sketch of the 595 bp DNA containing one closed end and one open end.