Figure 6. Analysis of mRNA expression using qPCR of four genes found downregulated with SSH.

Total RNA was extracted from pepper leaves with ABA spray or water, subsequently 72 h after foliar application exposed for 48 h to 6°C. SE was calculated based on three biological replicates. Closed circle: ABA-pretreated samples under room temperature; open circle: ABA-sprayed samples prior to chilling stress; triangle down: water-sprayed samples prior to chilling stress. The level of ubiquitin was used as an internal reference gene.