Figure 6. Effect of media change post transfection on HIV-1 virion infectivity.

The virus was produced by transfection of 293T cells using 1.0 μg pNL4-3E⁻ plasmid and 0.2 μg pcDNA3.1REC, and virions were collected at 18 and 48 hours post transfection. The differences in infectivity were confirmed by the Paired-sample t-test. Error bars are standard deviations from five independent replicates of the same experiments.