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. 2013 Jun 18;8(6):e67170. doi: 10.1371/journal.pone.0067170

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© 2013 Kim, et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panels (A), (B) and (C) show the infectivity, C_i.p. and C_p.p. of HIV-1 virions tagged with EGFP-Vpr, as a function of harvest time post transfection, with media change six hours post transfection (filled circles) and without (open circles). Panel (D) shows the infectivity of free EGFP virions as a function of the time of media change post transfection. The virus was collected 24 hours post transfection. Error bars are standard deviations from three independent replicates of the same experiments.