Table 2. Deficiency in ArgEF or CpcA but not ArgB decreases the cellular ornithine content.

	5 mM arginine	1 mM arginine	1 mM arginine	0.2 mM arginine
aa	ΔargEF/ΔakuB	ΔargEF/ΔakuB	ΔargB /Af293	ΔargB /Af293	ΔcpcA /D141
Ala	0.86	0.90	1.30	1.51	0.52
Arg	0.94	0.97	1.09	0.56	0.93
Asn	0.98	1.00	0.93	1.23	0.82
Asp	0.97	0.85	0.76	1.15	0.73
Gln	1.03	0.99	0.91	0.76	1.49
Glu	1.21	1.26	0.90	1.09	0.84
Gly	0.69	0.60	1.14	1.36	0.90
His	0.79	1.36	1.07	1.37	0.90
Ile	0.91	0.88	1.28	1.93	0.69
Leu	0.68	0.59	1.15	1.72	0.58
Lys	0.77	0.72	0.80	1.10	0.67
Met	0.62	0.75	0.85	1.58	0.86
Orn	0.15	0.02	1.55	3.12	0.45
Phe	0.82	0.73	1.23	2.00	0.56
Ser	0.99	0.97	0.92	1.33	0.82
Thr	1.00	1.07	0.99	1.48	0.61
Trp	0.51	0.43	1.31	1.58	0.40
Tyr	0.78	0.63	1.14	2.61	0.59
Val	0.78	0.79	1.41	2.01	0.59

ΔakuB and ΔargEF were supplemented either with 5 or 1 mM arginine; Af293 and ΔargB were supplemented either with 1 or 0.2 mM arginine. Individual amino acid pools are given in % of the total free amino acids and normalized to that of the respective wt. Amino acid pools up- or down-regulated >1.5- and >3-fold in mutant strain versus wt are in bold and bold, respectively. Given values represent the mean of three biological replicates, the standard deviations are given in Table S4.