Table 3. Impact of deficiency in ArgEF, ArgB, HapX and CpcA on the cellular spermidine content.
| spermidine nmol/mg dw | ratio mutant/wt | ||
| ΔakuB | ΔargEF | ||
| +Fe | 10.66±0.00 | 8.93±1.31 | 0.84 |
| −Fe | 4.18±0.14 | 3.07±0.06 | 0.73 |
| ratio –Fe/+Fe | 0.39 | 0.34 | |
| Af293 | ΔargB | ||
| +Fe | 9.66±0.62 | 11.17±2.26 | 1.16 |
| −Fe | 5.32±0.49 | 5.09±0.19 | 0.96 |
| ratio –Fe/+Fe | 0.55 | 0.46 | |
| D141 | ΔcpcA | ||
| +Fe | 8.95±0.33 | 14.83±0.18 | 1.66 |
| −Fe | 3.82±0.07 | 5.29±0.13 | 1.38 |
| ratio –Fe/+Fe | 0.43 | 0.36 | |
| wt | ΔhapX | ||
| +Fe | 8.25±1.51 | 8.71±0.91 | 1.06 |
| −Fe | 2.95±0.46 | 17.39±1.02 | 5.89 |
| ratio –Fe/+Fe | 0.36 | 2.00 | |
Cultures of ΔakuB, ΔargEF, Af293 and ΔargB were supplemented with 1 mM arginine. The values are the mean ± standard deviation of three biological replicates.