Figure 5.

Depletion of Tregs does not provoke changes in the levels of IL-1b, gp130, TNF-α, ROR-γτ, or CCL5. In samples from Foxp3.LuciDTR-4 or wild type animals treated with DT by daily application starting on day 2 and further mated allogeneically, inflammation markers were measured (n = 4–6/group) in uterine tissue by qPCR. Levels of IL-1b (A), gp130 (B), TNF-α (C), RORγτ (D), and CCL5 (E) mRNA were comparable in mice depleted of Tregs and DT-treated controls. Data are expressed as single dot plots with medians and were analyzed by Mann–Whitney-U test. No statistically significant differences were observed between both groups in any of the molecules.