FIG. 4.

Examples of HexonAM1-mediated prevention of nicotine-induced hypolocomotor activity. Assessments of distance traveled over 15 min in nicotine-sensitized and PBS-sensitized mice were compared for nonimmunized and HexonAM1-immunized mice over 5 weeks with frequent challenges of nicotine or PBS. (A–D) Examples of cumulative distance traveled assessed as a function of time post-administration of PBS or nicotine. (A) Fourth nicotine challenge (day 74, 10 weeks postsensitization); (B) fifth nicotine challenge (day 78, 11 weeks postsensitization); (C) seventh nicotine challenge (day 88, 12 weeks postsensitization); and (D) eighth nicotine challenge (day 92, 13 weeks postsensitization).