FIG. 3.

AAV2/9-associated stable transgene expression in rat salivary glands in vivo. (A–C) Biphotonic imaging of GFP in salivary glands at an expression interval of 5 weeks (n=3 each) or 15 weeks (n=4 each) after instillation of lactated Ringer's solution or AAV2/9-GFP (10¹¹ vg/gland). (D–F) Histological evaluation of hematoxylin and eosin-stained salivary gland sections, and (G–I) immunohistochemical localization of GFP at 5 and 15 weeks after delivery of lactated Ringers or AAV2/9-GFP (10¹¹ vg/gland). Nearly 25–50% of ductal cells were immunostained at moderate (score 2) intensity. Magnification, 200×. (J) Western blotting of submandibular gland tissue lysates with antibodies to TLK1 and β-actin at 5 and 15 weeks after retroductal delivery of lactated Ringers or AAV2/9-TLK1B (10¹¹ vg/gland).