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. 2013 Mar 11;4(6):940–951. doi: 10.1021/cn3002202

Figure 2.

Figure 2

D3 receptor N-terminus and TM4 are not sufficient to impart quinpirole- or PD128907-induced tolerance. Representative whole cell voltage clamp recordings obtained from AtT-20 cells stably expressing either the D2S chimeric receptor with the N-terminus region of the D3 receptor (A) or the D3 chimeric receptor with the N-terminus region of D2S receptor (B). The cells were held at −65 mV and inward GIRK currents in 30K-ES induced by 100 nM quinpirole (gray) or 100 nM PD128907 (black) measured. The agonists were applied for ∼60 s at indicated times. Tolerance property defined as the ratio of second/first agonist-induced GIRK response was determined for quinpirole (C) and PD128907 (D) in AtT-20 cells transiently transfected with either wild type D3 or D2S receptors or various chimeric receptors. Quinpirole- and PD128907-induced tolerance was significantly different only between wild type D3 receptor and all wild type and chimericD2 receptors; *P < 0.01, ANOVA (Holm’s-Sidak post hoc test). The bars represent the mean values ± SEM (n = 10–12 cells).