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. 2013 Mar 11;4(6):940–951. doi: 10.1021/cn3002202

Figure 3.

Figure 3

D3 receptor EC2 domain specifically imparts PD128907-induced tolerance to the D2D3IL123T-TM4 chimeric receptor. Representative whole cell voltage clamp recordings in AtT-20 cell transiently transfected with either the D2D3IL123T-TM4-EC1 (A) or D2D3IL123T-TM4-EC2 (B) chimeric receptors. The cells were held at −65 mV and inward GIRK currents in 30K-ES induced by 100 nM quinpirole (gray) or 100 nM PD128907 (black) measured. The agonists were applied for ∼60 s at indicated times. Tolerance property defined as the ratio of second/first agonist-induced GIRK response was determined for quinpirole (C) and PD128907 (D) in AtT-20 cells transiently transfected with either wild type D3 or D2S receptors or various chimeric receptors. Quinpirole- and PD128907-induced tolerance was significantly different between wild type D3 receptor and all wild type and chimeric D2 receptors; *P < 0.01, ANOVA (Holm’s-Sidak post hoc test). In addition, PD128907-induced tolerance was significantly different between the D2D3IL12T-TM4-EC2 chimeric receptor and all other receptors shown in panel D; #P < 0.01, ANOVA (Holm’s-Sidak post hoc test). The bars represent the mean values ± SEM (n = 10–12 cells).