Table 1.
Analytical Specificity of the UniProbe and Biothreat-specific Probes
| Clinically Relevant Organisms | UniProbe | Biothreat-specific Probes
|
||
|---|---|---|---|---|
| B. anthracis | Y. pestis | F. tularensis | ||
| Serratia marcescens (ATCC 8101) | + | − | − | − |
| Pseudomonas aeruginosa (ATCC 10145) | + | − | − | − |
| Staphylococcus epidermidis (ATCC 12228) | + | − | − | − |
| Staphylococcus aureus (ATCC 25923) | + | − | − | − |
| Corynebacterium sp.* | + | − | − | − |
| Enterococcus fecalis (ATCC 29212) | + | − | − | − |
| Streptococcus viridans (ATCC 10556) | + | − | − | − |
| C. albicans (ATCC 90029) | − | − | − | − |
| Proteus mirabilis* | + | − | − | − |
| Enterobacter sp.* | + | − | − | − |
| Acinetobacter sp. (ATCC 5459) | + | − | − | − |
| Streptococcus pyogenes* | + | − | − | − |
| Micrococcus sp. (ATCC 14396) | + | − | − | − |
| Neisseria meningitidis (ATCC 6250) | + | − | − | − |
| Listeria monocytogenes (ATCC 7648) | + | − | − | − |
| Legionella pneumoniae (ATCC 33495) | + | − | − | − |
| Haemophilus influenzae (ATCC 49247) | + | − | − | − |
| Streptococcus agalactiae (ATCC 13813) | + | − | − | − |
| Escherichia coli (ATCC 25927) | + | − | − | − |
| Streptococcus pneumoniae (ATCC 49619) | + | − | − | − |
| Citrobacter freundii* | + | − | − | − |
| Salmonella sp. (ATCC 31194) | + | − | − | − |
| BT-related organisms | ||||
| B. anthracis† | + | + | − | − |
| Bacillus subtilis* | + | + | − | − |
| Bacillus cereus* | + | + | − | − |
| Y. pestis (P14−) | + | − | + | − |
| F. tularensis (LVSB) | + | − | − | + |
| Yersinia pseudotuberculosis (PB1/+) | + | − | + | − |
| Francisella filomiragia (GAO1–2810)‡ | + | − | − | + |
| Coxiella burnetii§ | + | − | − | − |
| Brucella ovis¶ | + | − | − | − |
Note: + = indicate detected by probe; − = indicate not detected by probe.
ATCC = American Type Culture Collection (http://www.atcc.org/Home.cfm), Manassas, VA; LVSB = live vaccine strain type; PB1/+ = a wild-type strain; P14− = depigmented and virulence plasmid pCD1-negative.
Clinical isolate.
Inactivated nonpathogenic strain.14
GAO1-2810 = nonpathogenic strain obtained from Centers for Disease Control and Prevention, Fort Collins, CO, via the Walter Reed Army Medical Hospital, Washington, DC.
Coxiella burnetii DNA from Steve Dumler, MD, Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD.
Brucella ovis DNA obtained from Joany Jackman, PhD, Applied Physical Laboratory, Johns Hopkins University, Baltimore, MD.