Table 1.

Primer and Probe Sequences

Target Organism	Probe Sequence
Forward primer	5′-TGGAGCATGTGGTTTAATTCGA-3′
Reverse primer	5′-TGCGGGACTTAACCCAACA-3′
Uniprobe	5′-VIC-CACGAGCTGACGACARCCATGCA-3′-MGB
Gram-negative organisms	5′-VIC-ACAGGTGCTGCATGGCTGTCGTCAGCT-3′-MGB
N. meningitidis	5′-VIC-TCCGTCTCCGGAGGATTCCGTAC-3′-MGB
H. influenzae	5′FAM AAGGCACAAGCTCATCTCTGAGCTCTTCTTAGG 3′-MGB
E. coli	5′-FAM-ACATTCTCATCTCTGAAAACTTCCGTGGATGTC-3′-MGB
Gram-positive organisms	5′-FAM-AGGTGGTGCATGGTTGTCGTCAGC-3′-MGB
S. pneumoniae	5′-FAM-CCTTTGACAACTCTAGAGATAGAGCCTTCCC-3′-MGB
L. monocytogenes	5′TET-AAGGGAAAGCTCTGTCTCCAGAGTGGTCAA-3′-MGB
S. epidermidis	5′-TET-AAAACTCTATCTCTAGAGGGGCTAGAGGATGTCAAG-3′-MGB