Fig. 3.
Virtual Northern cDNA blotting assay indicating expression of PM3033 transcripts in sea lamprey larvae. a Ethidium bromide staining of cDNA samples after 1% agarose gel electrophoresis. bxs Autoradiogram after hybridization of PM3033 (top) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, bottom) cDNA sequences to the blotted cDNA from (a). Parallel hybridization with a probe complementing PM3601 failed to demonstrate this effect (data not shown). Identities of samples are indicated at the top. Typh Typhlosole, lymph lymphocyte-like cells, Lg large, Sm small, the arrow indicates the relative position of the PM3033 transcript. Approximate molecular sizes are indicated to the right of each image. The GAPDH probe hybridizes to two major transcripts, approximately 1.5 and 1.7 kilobases (kb) in length