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. 2013 Jul;15(7):749–760. doi: 10.1593/neo.121956

Figure 5.

Figure 5

Stable Sh3gl2 silencing increases phosphorylation of SFKs and STAT3. Lysates of control and Sh3gl2-silenced cells treated without (A) or with (B) 10 nM EGF for 10 minutes were analyzed using a phospho-kinase proteome array. Graphs and tables indicate phospho-proteins that were altered by >1.5-fold in response to Sh3gl2 silencing. Representative images of phospho-array blots from each condition are shown. (C) Independent lysates immunoblotted with the indicated antibodies revealed enhanced phosphorylation of SFKs at Y416 (or corresponding conserved residue) and STAT3 at Y705. (D) Control and Sh3gl2-silenced RT4 cells were treated with vehicle (DMSO) or 0.125 µM saracatinib for 5 days and cell number determined by biomass assay. Graphs illustrate absorbance on day 5 expressed as a percentage of absorbance on day 0, and data are the means ± SD of two values for DMSO and six values for saracatinib. Data are representative of at least two independent trials. *P < .05 compared to DMSO-treated condition.