Figure 5.

Downstream effects of siRNA-mediated Pim-1 knockdown or Pim-1 inhibition in HCT-116 colon carcinoma cells. (A) Upon Pim-1 knockdown, levels of Thr¹⁴⁵-phosphorylated p21 are markedly decreased (left), while overall p21 expression remains unchanged as determined on mRNA levels (center). Downstream of Pim-1, survivin mRNA, and protein levels are decreased (right). Western blots show data from a representative experiment. Since the same lysates were used, one loading control is shown. (B) Analysis of various proteins involved in signal transduction pathways related to cell survival and adhesion. Differences in expression levels are observed upon Pim-1 knockdown (right bands) compared to negative controls. For details, see text. (C) Effects of KH-CARB-13-mediated Pim-1 inhibitor on downstream signaling molecules confirm knockdown results. (D) Alterations in the levels of proapoptotic and antiapoptotic proteins upon Pim-1 knockdown.