Figure 6.

hD1R enhanced the recruitment of perivascular cells. U87 cells were inoculated s.c. in nude mice, and the mice were treated with PBS or hD1R. (A–C) Tumors were stained with anti-CD31 (green) and anti-NG2 (red). The NG2⁺ areas (B) and the ratio of NG2⁺ areas versus CD31⁺ areas (C) were compared. (D, E) On the last day of the experiments, mice were injected with lectin (green) through the tail vein. Tumors were dissected 30 minutes after the injection and were stained with anti-CD31 (red). The percentage of the perfused vessels (lectin and CD31 double positive) was counted and compared (E). Bars, means ± SD. *P < .05, **P < .01, ***P < .001, n = 8.