Cell characterization. A and B, Comparative characterization of GH, PRL, SSTR1, and SSTR2 gene expression in untreated WT GC and GH3 cells grown in DMEM enriched with 10% FBS. RNA was collected, and TaqMan qRT PCR was performed. C, Rat GH gene expression in WT GC cells treated with octreotide or vehicle (100 nM; daily for 3 consecutive days), after which RNA was collected and analyzed by TaqMan qRT PCR. D, Rat GH peptide levels measured by RIA in WT GC medium with or without octreotide (100 nM). ***, P < .001; ****, P < .0001. NT, not treated.