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. 2013 May 21;154(7):2399–2409. doi: 10.1210/en.2013-1132

Figure 2.

Figure 2.

Characterization of stable GC transfectants for ligand-dependent and -independent SSTR2 function. A, SSTR2 mRNA expression in GpCon and GpSSTR2WT cells and pooled male rat pituitary glands (n = 3). Cells were plated for 48 hours after which RNA collected, and rat pituitary glands were harvested immediately after sacrifice and preserved in RNAlater. Samples were analyzed simultaneously using SSTR2 TaqMan qRT PCR assays. A human SSTR2 TaqMan assay that recognizes both rodent and human SSTR2 was used. B, SRIF inhibition of intracellular cAMP levels in GpCon and GpSSTR2WT cells cotreated with IBMX and forskolin (1 μM) in DMEM supplemented with 0.3% BSA for 30 min. C, Baseline intracellular cAMP in untreated and IBMX (1 mM)–treated in GpCon and GpSSTR2WT cells. Cells were then lysed for intracellular cAMP measurements with the LANCE cAMP assay kit. NT, not treated.