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. Author manuscript; available in PMC: 2013 Jun 21.
Published in final edited form as: Immunity. 2002 Aug;17(2):211–220. doi: 10.1016/s1074-7613(02)00365-5

Figure 2. DT Sensitivity of the DC Compartment in DTR Transgenic Mice.

Figure 2

(A) Flow cytometric analysis of spleen cells of wt and DTR-transgenic (line 57) FVB/N mice 24 hr after injection of 100 ng DT or control vehicle.

(B) Analysis of splenic APC populations of homozygous CD11c-DTR transgenic mice (line 57) after i.p. injection of 100 ng DT(day 0). Data represent mean of two mice per time point and are representative of two independent experiments. B cells are represented by squares and defined as being CD19+; macrophages are represented by triangles and defined as being F4/80+; DC are presented by circles and defined as being CD11c+. Open squares represent total splenocyte counts. Dot blots below the graph show a representative flow cytometric analysis of two time points.

(C) Mixed leukocyte reaction with 8 × 105 stimulator splenocytes (FVB/N; H2q) and 105 responder T cells (C57BL/6; H2b). Data are representative of three experiments with similar results. Note that the MLR was also abrogated by addition of DT during the culture resulting in in vitro DC depletion.