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. Author manuscript; available in PMC: 2013 Jun 21.
Published in final edited form as: Immunity. 2002 Aug;17(2):211–220. doi: 10.1016/s1074-7613(02)00365-5

Figure 4. DC Dependence of Classical and Nonclassical MHC-Restricted Anti-Listeria CTL Responses.

Figure 4

(A) Flow cytometric analysis of splenocyte of nontransgenic and DTR transgenic (line 57) littermate BALB/c mice 10 hr after DT injection (4 ng/g body weight).

(B) Flow cytometric analysis of CFSE-labeled WP11.12 CD8+ T cell graft (106 cells) in DT-treated nontransgenic and DTR transgenic (line 57) BALB/c mice 4 days after i.v. injection of 2000 live L. monocytogenes. Histograms represent cells gated according to scatter, CD8, and Vβ 8 expression as indicated in dot plots. Note that the large CFSE− peak represents host Vβ 8+ CD8 T cells. Dead cells were excluded by PI staining.

(C) Flow cytometric analysis of CFSE-labeled C10.4 CD8+ T cell graft (Thy1.1, 106 cells) in spleens of DT-treated DTR transgenic (line 57) and nontransgenic littermate CB6F1 mice (Thy1.2) 4 days after i.v. injection of 2000 live L. monocytogenes. Histograms represent cells gated according to scatter, CD8, and Thy1.1 expression as indicated in dot plots. Dead cells were excluded by PI staining.