FIG. 2.
Detection of staphylococcal superantigen genes from CNS isolates from bovine intramammary infections by multiplex PCR. The mixtures of genomic DNAs from 5 S. aureus reference strains were used for multiplex PCR as a positive control for each primer sets. Genomic DNA extracted from S. aureus RN4220 was used as a negative control for multiplex PCR. Results shown are representative data obtained from various species of bovine IMI CNS isolates harboring SAg genes. (A) primer set 1 and 2, (B) primer set 3 and 4; Lane M: 100 bp DNA ladder.
